Physiochemical Evaluation of Low Molecular Weight Protamine Modified Hepatitis B Surface Antigen Nanoparticles for Mucosal Immunization
نویسنده
چکیده
In this study, low molecular weight heparin modified hepatitis B surface antigen (HBsAg) were prepared to evaluate the effect of coating material for nasal vaccine delivery. The developed formulations were characterized for size, zeta potential, entrapment efficiency, and mucin adsorption ability. Plain HBsAg nanoparticles demonstrated negative zeta potential and LMWP coated nanoparticles showed higher positive zeta potential. When Plain HBsAg and LMWP coated HBsAg were administered intranasally to mice, it was observed that LMWP coated particles showed a markedly increased anti-HBsAg titer as compared to plain HBsAg nanoparticles.
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